Objective To investigate the effects of serum containing Fuzheng Kangai Decoction (FZKAD) on the proliferation, apoptosis, and cell cycle progression in human ovarian carcinoma HO-8910PM cells. Methods Forty Sprague-Dawley rats were divided randomly into control and low-dose (4. 725 g/ kg), medium-dose (9. 45 g/ kg), and high-dose (18. 9 g/ kg) FZKAD groups. After 7 days of intragastric administration, serum containing FZKAD was prepared and used for the culture of HO-8910PM cells. Cell proliferation was detected by MTT assay, colony-formation ability was detected by clone formation assay, apoptosis was detected by flow cytometry using Annexin V-fluorescein isothiocyanate/propidium iodide double-staining, cell cycle progression was detected by flow cytometry using propidium iodide single staining, and protein expression levels were detected by Western blot. Results There was no significant difference in cell survival rates among the low-dose, medium-dose, and control groups after 24 h ( P>0. 05). However, cell survival rates in the other groups were significantly decreased after 24, 48 and 72 h (P<0. 05 or P<0. 01), and the colony-formation abilities were also significantly decreased in each dosage group (P<0. 01). Compared with the control group, the apoptosis rates and protein expression levels of P53 and Bax were significantly increased in the low-dose, medium-dose, and highdose groups (P<0. 05 or P<0. 01), while the protein expression level of Bcl-2 was decreased (P<0. 01). The percentage of cells in G₀ / G₁ stage was significantly increased in the low-dose, medium-dose, and high-dose groups compared with the control group (P<0. 05 or P<0. 01), while the percentages of cells in S and G₂ / M stages and protein expression levels of cyclin D1 and cyclin-dependent kinases 4 and 6 were decreased (P<0. 05 or P<0. 01). Conclusions Serum containing FZKAD can inhibit the proliferation of human ovarian cancer HO-8910PM cells, related to the induction of apoptosis and cell cycle arrest.