精氨酸加压素 1b 受体敲除对雌性大鼠焦虑和社交行为的影响
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中国医学科学院基础医学研究所 医学分子生物学国家重点实验室,北京 100005

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R-33

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Effects of arginine vasopressin 1b receptor knockout on anxiety behavior and social behavior in female rats
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State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China

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    摘要:

    目的 探究精氨酸加压素 1b 受体(arginine vasopressin 1b receptor,AvpR1b)基因敲除对雌性大鼠血浆皮质酮含量、焦虑和社交行为的影响。 方法 以精氨酸加压素 1b 受体敲除(AvpR1bKO )和野生型(wild type,WT) 的 SD 雌性大鼠为研究对象,首先采用原位杂交技术检测 WT 和 AvpR1bKO 大鼠大脑室旁核( paraventricular nucleus, PVN)与视上核(supraoptic nucleus,SON)中精氨酸加压素 1b 受体的表达水平;采用高效液相色谱串联高分辨质谱分析 WT 和 AvpR1bKO 大鼠静息状态下血浆皮质酮含量;通过旷场实验中 WT 和 AvpR1bKO 大鼠进入中心区域的时间、次数,以及高架十字迷宫实验中 WT 和 AvpR1bKO 大鼠进入开放臂的时间和次数来评价大鼠的焦虑水平;通过三箱实验中 WT 和 AvpR1bKO 大鼠探索陌生鼠的时间来评价大鼠社交能力;并根据 WT 和 AvpR1bKO 大鼠探索熟悉程度不同陌生大鼠的时间来评价大鼠社交新颖性。 结果 在 AvpR1bKO 大鼠室旁核与视上核脑区精氨酸加压素 1b 受体均无表达;静息状态下,AvpR1bKO 大鼠血浆中皮质酮含量较 WT 大鼠无差异(P>0.05);旷场实验中 AvpR1bKO 大鼠进入中心区域的时间、次数与 WT 大鼠无差异(P>0.05);高架实验中 AvpR1bKO 大鼠进入开放臂区域的时间、次数与 WT 大鼠无差异(P>0.05);在三箱实验中 AvpR1bKO 大鼠的社交能力指数与 WT 大鼠的社交能力指数无显著差异(P>0.05);同时 AvpR1bKO 大鼠的社会新颖指数与 WT 大鼠的社会新颖指数无显著差异(P>0.05)。 结论 精氨酸加压素 1b 受体敲除不影响雌性大鼠静息状态下血浆中皮质酮含量、焦虑水平、社交能力以及社交新颖性。

    Abstract:

    Objective To investigate the effects of arginine vasopressin 1b receptor (Avpr1b) gene knockout on the plasma corticosterone level, anxiety, and social behaviors in female rats. Methods Female AvpR1b knockout (AvpR1bKO ) and wildtype (WT) SD rats were used. First, the expression levels of Avpr1b in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of the brains of WT and AvpR1bKO rats were examined by performing in situ hybridization. The basal plasma corticosterone levels of WT and AvpR1bKO rats were then analyzed by conducting high- performance liquid chromatography tandem high-resolution mass spectrometry. Additionally, the anxiety levels of rats were evaluated by using an open field test and elevated plus maze test. Finally, the social ability and social novelty of WT and AvpR1bKO rats were measured by performing a three-chamber experiment. Results There was no expression of AvpR1b in paraventricular nucleus or supraoptic nucleus of AvpR1bKO rats. The basal plasma corticosterone level of AvpR1bKO rats was not significantly different from that of WT rats (P>0.05). The duration and bouts of AvpR1bKO rats entering the central area in the open field experiment were not different from those of WT rats ( P> 0.05). Similarly, the duration and bouts of AvpR1bKO rats entering the open arm area in the elevated plus maze were not different from those of WT rats (P>0.05). In the social interaction experiment, the socialbility index of AvpR1bKO rats was not different from that of WT rats (P>0.05). In the social novelty experiment, the social novelty index of AvpR1bKO rats was not different from that of WT rats ( P> 0.05). Conclusions A knockout of AvpR1b in female SD rats does not affect their basal plasma corticosterone level, anxiety level, sociability, or interest in social novelty.

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赵崟机,樊 圃.精氨酸加压素 1b 受体敲除对雌性大鼠焦虑和社交行为的影响[J].中国比较医学杂志,2022,32(6):69~75.

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  • 收稿日期:2022-01-21
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  • 在线发布日期: 2022-08-01
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