小鼠烧伤创面脓毒症模型的建立与评价
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陆军军医大学第一附属医院临床医学研究中心,重庆 400038

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R-33

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Establishment and evaluation of a murine burn wound sepsis model
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Clinical Medicine Research Center, First Affiliated Hospital, Army Medical University, Chongqing 400038, China

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    摘要:

    目的 建立一种稳定的小鼠烧伤创面脓毒症模型,检测其病理生理指标,为烧伤脓毒症相关研究提供标准化的动物模型。 方法 将小鼠背部侵入 90℃水面接触 8 s 建立小鼠Ⅲ度烫伤模型,烫伤面积约 15% ~ 20%。 2 h 后痂下注射 100 μL 浓度为 0. 75×105 CFU/ mL 的铜绿假单胞菌(Pseudomonas aeruginosa,PA)造伤后创面脓毒症。 连续 12 d 观测记录小鼠的体重、采食量、生存率和一般状态指标。 在注射后 1、2、3、5、7 d 取小鼠外周血和肝组织,测定血清生化和炎症因子指标,观察肝组织病理改变。 结果 模型组建模后 12 d 的死亡率为 30. 2%。 血清生化指标中 ALT、AST、UREA 和 CREA 均较建模前显著上升(P<0. 05 或 P<0. 01),TP 和 TBIL 指标无明显变化。 血清炎症指标中 TNF-α、IL-6 在建模后第 1 天升至最高值(P<0. 01),随后逐日递减,分别至第 3、7 天恢复至造模前水平。 肝组织病理切片结果显示在各个时相点均出现肝损伤表现。 结论 建立了操作简便可控、严重程度适宜的烧 伤脓毒症模型,符合烧伤脓毒症的病理生理特点,可为相关机制研究和药物研发提供标准化的实验动物模型。

    Abstract:

    Objective To establish a stable mouse burn wound sepsis model, determine its pathophysiological indicators, and provide a standardized animal model for the study of burn sepsis. Methods The back of a mouse was exposed for 8 s to water with a temperature of 90℃ to establish a third degree scald model, and the scald area was about 15% ~ 20%. Two hours later, 100 μL of Pseudomonas aeruginosa (PA) with a concentration of 0. 75×105 colony-forming units/ mL was injected under the eschar to create wound sepsis. The bodyweight, feed intake, survival rate and general status of the mice were observed and recorded for 12 days. Peripheral blood and liver tissues were collected from the mice on post-infection days 1, 2, 3, 5 and 7, the serum biochemical and inflammatory factors were measured, and the pathological changes of liver tissues were observed. Results The mortality of the model mice on the twelfth day post- infection was 30. 2%. The levels of the serum biochemical indexes ALT, AST, UREA and CREA were significantly increased from baseline (P<0.05 or P<0.01), whereas the levels of TP and TBIL exhibited no obvious changes. The serum inflammatory indexes TNF-α and IL-6 increased to their highest values on the first day after infection (P<0.01) and then decreased daily, recovering to their baseline levels on the third and seventh days post-infection, respectively. The histopathological sections of liver revealed liver injury at all tested timepoints. Conclusions A simple burn sepsis model with a manageable protocol and appropriate severity was established; the model characteristics are in line with the pathophysiological characteristics of burn sepsis; and this approach can provide a standardized experimental animal model both for relevant mechanism research and for drug research and development.

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范仕郡,吴 丹,夏 林,魏 燕,陈 倩,彭 曦.小鼠烧伤创面脓毒症模型的建立与评价[J].中国比较医学杂志,2022,32(6):7~13.

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  • 收稿日期:2021-08-04
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  • 在线发布日期: 2022-08-01
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