Objective To summarize the initial operation and management of the laboratory animal barrier housing facilities at Central South University. Methods Based on practice, a series of measures have been worked out to improve the management of personnel, animals and items, implement of rules and regulations,and disinfection and purification of the facilities during the operation of barrier system. Results Through strictly carrying out the management measures, we have ensured the quality of laboratory animals and have established a scientific, high-quality and efficient service platform of laboratory animals to serve the teaching and research at Central South University. Conclusions Only through strict management, we can ensure the normal operation of the laboratory animal barrier housing facilities.