ObjectiveTo investigate the pathogenesis of nonalcoholic fatty liver disease(NAFLD), provide a reference for the evaluation of fatty liver therapeutic effect, the liver function, lipid metabolism,insulin resistance and liver apoptosis of NAFLD rats established by high cholesterol feeding were continuously monitored from 4~16 weeks. Methods 40 NAFLD rats established by high cholesterol feeding were randomly divided into the 4,8,2,6 weeks model group and a group of normal rats were set up for control, the serum GLU、CHO、TG、HDL、LDL、GPT、GOT and insulin were tested, the liver apoptosis and pathological changes were observed, then analyzed the effect of the liver function, lipid metabolism,insulin resistance and apoptosis on the pathological changes in non-alcoholic fatty liver rats. ResultsAfter 4 weeks of high cholesterol feeding, the rats had got the liver injury, disorder of lipid metabolism, insulin resistance. Liver cell apoptosis were increased obviously, and pathological changes were characterized by cell steatosis and inflammatory, the lesions became more serious with time went on. ConclusionsRats established by high cholesterol feeding could get the nonalcoholic fatty liver disease and the lesions became more serious with time over during 4~16 weeks. Lipid metabolism disorder and insulin resistance were the important cause of liver cell steatosis and inflammatory. The model we established is suitable for the evaluation of fatty liver therapeutic effect.